|Occupation||Littlest Pet Shop owner;
Retired Kung Fu Quiltingmaster
|Residence||Littlest Pet Shop|
|Debut||Blythe's Big Adventure Part One|
|Voiced by||Kathleen Barr|
Anna Twombly, often addressed simply as Mrs. Twombly or Mrs. T, is the owner of the Littlest Pet Shop. She hires Blythe as a part-time worker after her fashion expertise saved the Shop. She's also a collector of very rare door knobs.